Browse by author
Lookup NU author(s): Dr Gabriele Saretzki, Professor Thomas von Zglinicki
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Human foreskin BJ fibroblasts are well protected against oxidative stress as shown by their low intracellular peroxide content, low levels of protein carbonyls, and low steady-state lipofuscin content as compared to other primary human fibroblasts. This correlates with a long replicative life span of the parental cells of about 90 population doublings and a telomere-shortening rate of only 15-20 bp/PD. This value might define the upper limit of a telomere-shortening rate that can still be explained by the end replication problem alone. In BJ clones immortalized by transfection with hTERT, the catalytic subunit of telomerase, the same telomere-shortening rate as in parental cells is observed over a long time despite strong telomerase activity. Hyperoxia, which induces oxidative stress and accelerates telomere shortening in a variety of human fibroblast strains, does not do so in BJ cells. It is possible that the high antioxidative capacity of BJ cells, by minimizing the accumulation of genomic damage, is instrumental in the successful immortalization of these cells by telomerase. © 2001 Elsevier Science Inc.
Author(s): Saretzki G; Von Zglinicki T; Lorenz M; Sitte N; Metzkow S
Publication type: Article
Publication status: Published
Journal: Free Radical Biology and Medicine
Year: 2001
Volume: 31
Issue: 6
Pages: 824-831
ISSN (print): 0891-5849
ISSN (electronic): 1873-4596
Publisher: Elsevier
URL: http://dx.doi.org/10.1016/S0891-5849(01)00664-5
DOI: 10.1016/S0891-5849(01)00664-5
PubMed id: 11557321
Altmetrics provided by Altmetric
