<em>In vitro </em>generation of human tolerogenic monocyte-derived dendritic cells

Hilkens, CMU; Diboll, J; Cooke, F; Anderson, AE

doi:10.1007/978-1-0716-3135-5_31

In vitro generation of human tolerogenic monocyte-derived dendritic cells

Lookup NU author(s): Professor Catharien Hilkens ORCiD, Julie Diboll, Dr Fiona Cooke, Dr Amy Anderson ORCiD

Downloads

Accepted version [.pdf]

Licence

This is the authors' accepted manuscript of an authored book that has been published in its final definitive form by Humana/Springer, 2023.

For re-use rights please refer to the publisher's terms and conditions.

Abstract

Human monocyte-derived dendritic cells (moDC) are commonly used as a research tool to investigate interactions between antigen-presenting cells and T cells. Generation of these cells involves the isolation of CD14⁺ monocytes from peripheral blood and their in vitro differentiation into immature moDC by the cytokines GM-CSF and IL-4. Their functional characteristics can then be manipulated by maturing these cells with a cocktail of agents, which can be tailored to induce either immune activating or tolerogenic properties. Here, we describe a protocol for the generation of moDC with stable tolerogenic function, referred to as tolerogenic dendritic cells. These cells have been developed as an immunotherapeutic tool for the treatment of autoimmune disease, but have also proven useful to dissect mechanisms of T cell tolerance induction in vitro.