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Lookup NU author(s): Yaobo Xu, Dr Matthew Barter, Dr Daniel Swan, Dr Kenneth RankinORCiD, Emeritus Professor Drew Rowan, Dr Mauro Santibanez Koref, Professor John LoughlinORCiD, Professor David YoungORCiD
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Objective: To define for the first time the transcriptomes of normal and end-stage osteoarthritis (OA) hip cartilage. Materials and methods: RNA was isolated from cartilage within 2 h of joint replacement surgery. Gene expression was analyzed using Agilent GeneSpring GX 11 following hybridization to Illumina Human HT-12 V3 microarrays. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was used to validate the expression of six genes identified by microarray as differentially expressed. Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) were used to investigate enriched functions or canonical pathways amongst differentially expressed genes respectively. Results: In total we identified 998 differentially expressed genes (fold change >= +/- 1.5, P-value <= 0.01) between neck of femur fracture (NOF) (n = 10) and OA hip (n = 9) patient cartilage. These differentially expressed genes were enriched within 71 canonical pathways. A comparison between a comparable knee dataset(20) only identified 229 genes similarly differentially expressed although remarkably 34 canonical pathways overlapped between experiments. Conclusions: This study is the first to report a comprehensive gene expression analysis of human hip OA cartilage compared to control (NOF) cartilage at the whole-genome level. Our differential gene expression dataset shows excellent correlation with similar defined studies using comparable tissue but reveals discord between hip and knee OA at the individual gene status but with commonality with regards the molecular pathways involved. (c) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Author(s): Xu Y, Barter MJ, Swan DC, Rankin KS, Rowan AD, Santibanez-Koref M, Loughlin J, Young DA
Publication type: Article
Publication status: Published
Journal: Osteoarthritis and Cartilage
Year: 2012
Volume: 20
Issue: 9
Pages: 1029-1038
Print publication date: 01/09/2012
ISSN (print): 1063-4584
ISSN (electronic): 1522-9653
Publisher: Elsevier Ltd
URL: http://dx.doi.org/10.1016/j.joca.2012.05.006
DOI: 10.1016/j.joca.2012.05.006
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