Toggle Main Menu Toggle Search

Open Access padlockePrints

Raised interleukin-17 is immunolocalised to neutrophils in cystic fibrosis lung disease

Lookup NU author(s): Dr Malcolm Brodlie, Dr Michael McKean, Dr Amy AndersonORCiD, Professor Catharien Hilkens, Professor Andrew FisherORCiD, Dr James Lordan, Professor Christopher WardORCiD

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Abstract Interleukin-17 is pivotal in orchestrating the activity of neutrophils. Neutrophilic inflammation is the dominant pathology in cystic fibrosis lung disease. We investigated interleukin-17 protein expression in the lower airway in cystic fibrosis, its cellular immuno-localisation and the effects of interleukin-17 on cystic fibrosis primary bronchial epithelial cells. Immunohistochemistry was performed on explanted cystic fibrosis lungs and compared to the non-suppurative condition pulmonary hypertension. Airway lavages and epithelial cultures were generated from explanted cystic fibrosis lungs. Immunoreactivity for interleukin-17 was significantly increased in the lower airway epithelium in cystic fibrosis (median 14.1%) compared to pulmonary hypertension (2.95%, P=0.0001). The number of cells staining positive for interleukin-17 in the lower airway mucosa was also increased (64 compared to 9/mm basement membrane, P=0.0005) and included both neutrophils in addition to mononuclear cells. Interleukin-17 was detectable in airway lavages from explanted cystic fibrosis lungs. Treatment of epithelial cultures with interleukin-17 increased production of interleukin-8, interleukin-6 and granulocyte macrophage colony-stimulating factor. In conclusion, immunoreactive interleukin-17 is raised in the lower airway of people with cystic fibrosis and localises to both neutrophils and mononuclear cells. Interleukin-17 increases production of pro-neutrophilic mediators by cystic fibrosis epithelial cells, suggesting potential for a positive feedback element in airway inflammation.


Publication metadata

Author(s): Brodlie M, McKean MC, Johnson GE, Anderson AE, Hilkens CM, Fisher AJ, Corris PA, Lordan JL, Ward C

Publication type: Article

Publication status: Published

Journal: European Respiratory Journal

Year: 2011

Volume: 37

Issue: 6

Pages: 1378-1385

Print publication date: 25/11/2010

ISSN (print): 0903-1936

ISSN (electronic): 1399-3003

Publisher: European Respiratory Society

URL: http://dx.doi.org/10.1183/09031936.00067110

DOI: 10.1183/09031936.00067110

PubMed id: 21109552


Altmetrics

Altmetrics provided by Altmetric


Share