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Lookup NU author(s): Professor Laura GreavesORCiD, Emeritus Professor Doug Turnbull
The mechanisms of how DNA mutations are fixed within the human gastrointestinal tract and how they spread are poorly understood and are hotly debated. It has been well documented that human colonic crypts are clonal units; one epithelial stem cell within the crypt becoming dominant and taking over the crypts' entire stem cell population - so called monoclonal conversion. Studies have revealed that crypts can exist as families and develop into patches. The questions have been how do such patches in the human colon develop? Does this have implications on how DNA mutations spread? We have previously shown that mitochondrial DNA ( mtDNA) mutations, which result in the deficiency of cytochrome c oxidase, are established within a single colonic crypt stem cell, resulting in a crypt with a mixed phenotype. Over time that mutated stem cell can take over the entire stem cell population resulting in a wholly-mutated crypt. We have furthered this research by showing that entirely cytochrome c oxidase - deficient crypts are able to divide by a process called crypt fission, to form two cytochrome c oxidase- deficient daughter crypts, each sharing the exact parental mtDNA mutation. Furthermore, patches of these crypts also possess a founder mtDNA mutation suggesting that fission repeats itself to form patches, which increase in size with age. Here, we hypothesize that this can be expanded into other areas of the gastrointestinal tract, especially the stomach, where there is a paucity of data regarding clonality and the spread of DNA mutations. We ask if these mutated crypts expand at a different rate to wild type ones. We also discuss the implications for the spread of potential carcinogenic mutations within the gut.
Author(s): McDonald SAC, Preston SL, Greaves LC, Leedham SJ, Lovell MA, Jankowski JAZ, Turnbull DM, Wright NA
Publication type: Article
Publication status: Published
Journal: Cell Cycle
Year: 2006
Volume: 5
Issue: 8
Pages: 808-811
Date deposited: 24/11/2010
ISSN (print): 1538-4101
ISSN (electronic): 1551-4005
Publisher: Landes Bioscience
URL: http://www.landesbioscience.com/journals/cc/article/2641/
