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Lookup NU author(s): Professor Karim Nayernia, Dr Jae Ho Lee, Dr Ingrid Ehrmann, Professor David Elliott
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Male gametes originate from a small population of spermatogonial stem cells (SSCs). These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male. Here, we developed a strategy for the establishment of SSC lines from embryonic stem (ES) cells. These cells are able to undergo meiosis, are able to generate haploid male gametes in vitro, and are functional, as shown by fertilization after intracytoplasmic injection into mouse oocytes. Resulting two-cell embryos were transferred into oviducts, and live mice were born. Six of seven animals developed to adult mice. This is a clear indication that male gametes derived in vitro from ES cells by this strategy are able to induce normal fertilization and development. Our approach provides an accessible in vitro model system for studies of mammalian gametogenesis, as well as for the development of new strategies for the generation of transgenic mice and treatment of infertility. © 2006 Elsevier Inc. All rights reserved.
Author(s): Nayernia K, Nolte J, Michelmann HW, Lee JH, Rathsack K, Drusenheimer N, Dev A, Wulf G, Ehrmann IE, Elliott DJ, Okpanyi V, Zechner U, Haaf T, Meinhardt A, Engel W
Publication type: Article
Publication status: Published
Journal: Developmental Cell
Year: 2006
Volume: 11
Issue: 1
Pages: 125-132
ISSN (print): 1534-5807
ISSN (electronic): 1878-1551
Publisher: Cell Press
URL: http://dx.doi.org/10.1016/j.devcel.2006.05.010
DOI: 10.1016/j.devcel.2006.05.010
PubMed id: 16824959
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