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Lookup NU author(s): Dr Carmen Martin-RuizORCiD, Professor Thomas von Zglinicki
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Fat depots vary in size, function, and potential contribution to disease. Since fat tissue turns over throughout life, preadipocyte characteristics could contribute to this regional variation. To address whether preadipocytes from different depots are distinct, we produced preadipocyte strains from single abdominal subcutaneous, mesenteric, and omental human preadipocytes by stably expressing human telomere reverse transcriptase (hTERT). These strains could be subcultured repeatedly and retained capacity for differentiation, while primary preadipocyte adipogenesis and replication declined with subculturing. Primary omental preadipocytes, in which telomeres were longest, replicated more slowly than mesenteric or abdominal subcutaneous preadipocytes. Even after 40 population doublings, replication, abundance of the rapidly replicating preadipocyte subtype, and resistance to tumor necrosis factor {alpha}-induced apoptosis were highest in subcutaneous, intermediate in mesenteric, and lowest in omental hTERT-expressing strains, as in primary preadipocytes. Subcutaneous hTERT-expressing strains accumulated more lipid and expressed more adipocyte fatty acid-binding protein (aP2), peroxisome proliferator-activated receptor {gamma}2, and CCAAT/enhancer-binding protein {alpha} than omental cells, as in primary preadipocytes, while hTERT abundance was similar. Thus, despite dividing 40 population doublings, hTERT strains derived from single preadipocytes retained fat depot-specific cell dynamic characteristics, consistent with heritable processes contributing to regional variation in fat tissue function. © 2006 by the American Diabetes Association.
Author(s): Tchkonia T, Giorgadze N, Pirtskhalava T, Thomou T, De Ponte M, Koo A, Forse RA, Chinnappan D, Martin-Ruiz C, Von Zglinicki T, Kirkland JL
Publication type: Article
Publication status: Published
Journal: Diabetes
Year: 2006
Volume: 55
Issue: 9
Pages: 2571-2578
ISSN (print): 0012-1797
ISSN (electronic): 1939-327X
Publisher: American Diabetes Association
URL: http://dx.doi.org/10.2337/db06-0540
DOI: 10.2337/db06-0540
PubMed id: 16936206
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