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Lookup NU author(s): Emeritus Professor Nick Europe-Finner
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Granulosa cells are essential for follicular development and corpus luteum formation and their functions are regulated by gonadotrophins through G protein-coupled receptors. The dominant second messenger pathway involves the stimulation of cyclic AMP formation by Gα(s)-linked receptors. In this paper we have investigated the expression of Gα(s) mRNA splice variants in relation to expression of Gα(s) protein isoforms in granulosa cells obtained from patients undergoing in vitro fertilization. We have carried out ribonuclease protection assays using cRNA riboprobes which are capable of detecting all Gα(s) mRNA isoforms as well as quantifying total amounts of Gα(s) mRNA. Granulosa cells express the message for Gα(s)-Large and Gα(s)-Small and the presence of two distinct protein products was confirmed by immunoblotting using the antibody RM/1. Moreover, the data show that a significant fraction of Gα(s)-Large and Gα(s)-Small mRNAs contain an extra CAG codon. This should generate proteins with an extra serine residue, resulting in Gα(s) variants with the consensus sequence of a protein kinase C phosphorylation site. These results highlight the possible interaction between different signalling pathways in the control of cAMP production and the need to investigate the relationship between Gα(s) variants and different adenylyl cyclase isozymes in patients with normal and abnormal ovarian function.
Author(s): Europe-Finner GN, Cartwright E, Bellinger J, Mardon HJ, Barlow DH, Lopez Bernal A
Publication type: Article
Publication status: Published
Journal: Journal of Molecular Endocrinology
Year: 1997
Volume: 18
Issue: 1
Pages: 27-35
Print publication date: 01/02/1997
ISSN (print): 0952-5041
ISSN (electronic): 1479-6813
Publisher: BioScientifica Ltd.
URL: http://dx.doi.org/10.1677/jme.0.0180027
DOI: 10.1677/jme.0.0180027
PubMed id: 9061604
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