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Lookup NU author(s): Dr Gabriele Saretzki, Professor Alexander Burkle
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024 by the authors. Chronic stress is associated with a higher risk for carcinogenesis as well as age-related diseases and immune dysfunction. There is evidence showing that psychological stress can contribute to premature immunosenescence. Therefore, the question arose whether chronic exposure to catecholamine could drive immune cells into senescence. Peripheral blood mononuclear cells were isolated from whole blood. After repeated ex vivo treatment with isoproterenol, an epinephrine analog, well-established senescence biomarkers were assessed. We found (i) DNA double-strand break induction, (ii) telomere shortening, (iii) failure to proliferate, (iv) higher senescence-associated β-galactosidase activity, (v) decreases in caspases 3 and 7 activity, and (vi) strong upregulation of the proteoglycan versican accompanied by increased cellular adhesion suggesting the induction of a senescence-like phenotype. These results emphasize the complexity of the effect of isoproterenol on multiple cellular processes and provide insights into the molecular mechanisms of stress leading to immunosenescence.
Author(s): Laumann M, Palombo P, Fieres J, Thomas M, Saretzki G, Burkle A, Moreno-Villanueva M
Publication type: Article
Publication status: Published
Journal: Biomolecules
Year: 2024
Volume: 14
Issue: 12
Online publication date: 28/11/2024
Acceptance date: 21/11/2024
Date deposited: 07/01/2025
ISSN (electronic): 2218-273X
Publisher: MDPI
URL: https://doi.org/10.3390/biom14121528
DOI: 10.3390/biom14121528
Data Access Statement: Data will be available upon request to Maria Moreno-Villanueva (maria.moreno-villanueva@uni-konstanz.de).
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