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Lookup NU author(s): Dan Salthouse, Peter Goulding, Sophie Reay, Emma Jackson, Professor Katarina NovakovicORCiD, Professor Catharien Hilkens, Dr Ana Ferreira-DuarteORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Copyright © 2024 Salthouse, Goulding, Reay, Jackson, Xu, Ahmed, Mearns-Spragg, Novakovic, Hilkens and Ferreira.Introduction: Collagen is extensively utilised in regenerative medicine due to its highly desirable properties. However, collagen is typically derived from mammalian sources, which poses several limitations, including high cost, potential risk of immunogenicity and transmission of infectious diseases, and ethical and religious constraints. Jellyfish-sourced type 0 collagen represents a safer and more environmentally sustainable alternative collagen source. Methods: Thus, we investigated the potential of jellyfish collagen-based hydrogels, obtained from Rhizostoma pulmo (R. pulmo) jellyfish, to be utilised in regenerative medicine. A variety of R. pulmo collagen hydrogels (RpCol hydrogels) were formed by adding a range of chemical crosslinking agents and their physicochemical and biological properties were characterised to assess their suitability for regenerative medicine applications. Results and Discussion: The characteristic chemical composition of RpCol was confirmed by Fourier-transform infrared spectroscopy (FTIR), and the degradation kinetics, morphological, and rheological properties of RpCol hydrogels were shown to be adaptable through the addition of specific chemical crosslinking agents. The endotoxin levels of RpCol were below the Food and Drug Administration (FDA) limit for medical devices, thus allowing the potential use of RpCol in vivo. 8-arm polyethylene glycol succinimidyl carboxyl methyl ester (PEG-SCM)-crosslinked RpCol hydrogels preserved the viability and induced a significant increase in the metabolic activity of immortalised human mesenchymal stem/stromal cells (TERT-hMSCs), therefore demonstrating their potential to be utilised in a wide range of regenerative medicine applications.
Author(s): Salthouse D, Goulding PD, Reay SL, Jackson EL, Xu C, Ahmed R, Mearns-Spragg A, Novakovic K, Hilkens CMU, Ferreira AM
Publication type: Article
Publication status: Published
Journal: Frontiers in Bioengineering and Biotechnology
Year: 2024
Volume: 12
Pages: epub ahead of print
Online publication date: 26/07/2024
Acceptance date: 12/07/2024
Date deposited: 19/08/2024
ISSN (electronic): 2296-4185
Publisher: Frontiers Media SA
URL: https://doi.org/10.3389/fbioe.2024.1391728
DOI: 10.3389/fbioe.2024.1391728
Data Access Statement: The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://data.ncl.ac.uk.
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