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An enhancer complex confers both high-level and cell-specific expression of the human type X collagen gene

Lookup NU author(s): Professor David YoungORCiD

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Abstract

Type X collagen expression is restricted to hypertrophic chondrocytes in the endochondral growth plate. Transient transfection of reporter constructs containing the human collagen X promoter into primary growth plate chondrocytes identified a cis-acting positive regulatory DNA element(s) that has cell-specific enhancer properties and binds a nuclear protein expressed specifically in growth plate chondrocytes. Functional disruption of this region results in a significant reduction in the activation of reporter gene transcription. The identified enhancer is a major element controlling both high-level and cell-specific expression of type X collagen gene.

Publication metadata

Author(s): Chambers D, Young DA, Howard C, Thomas JT, Boam DS, Grant ME, Wallis GA, Boot-Handford RP

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2002

Volume: 531

Issue: 3

Pages: 505-508

Print publication date: 20/11/2002

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/S0014-5793(02)03606-2

DOI: 10.1016/S0014-5793(02)03606-2

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