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Lookup NU author(s): Dr Anastasia Hepburn, Emma Curry, Dr Mohammad Moad, Laura WilsonORCiD, Parmveer Singh, Dr Adriana BuskinORCiD, Dr Luke GaughanORCiD, Professor Craig Robson, Professor Rakesh Heer
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2020 The Authors. STEM CELLS TRANSLATIONAL MEDICINE published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. Primary culture of human prostate organoids and patient-derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complimentary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease.
Author(s): Hepburn AC, Curry EL, Moad M, Steele RE, Franco OE, Wilson L, Singh P, Buskin A, Crawford SE, Gaughan L, Mills IG, Hayward SW, Robson CN, Heer R
Publication type: Article
Publication status: Published
Journal: Stem Cells Translational Medicine
Year: 2020
Volume: 9
Issue: 7
Pages: 734-745
Print publication date: 01/07/2020
Online publication date: 14/03/2020
Acceptance date: 29/01/2020
Date deposited: 18/05/2021
ISSN (print): 2157-6564
ISSN (electronic): 2157-6580
Publisher: John Wiley and Sons Ltd
URL: https://doi.org/10.1002/sctm.19-0286
DOI: 10.1002/sctm.19-0286
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