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Lookup NU author(s): Dr Matt WilcoxORCiD, Dr Peter Chater, Professor Jeffrey Pearson
© 2017 Future Medicine Ltd. Aim: Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene. Methods: Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase. With these synthesized derivatives, different nanoparticle formulations, including the cystic fibrosis transmembrane conductance regulator gene were prepared by ionic gelation. Results: A change from negative to positive ζ potential after enzymatic cleavage could be observed. Transfection studies with HEK-293 and Caco-2 cells showed transfection rates comparable to Lipofectamine 2000. Transfection efficiencies were significantly decreased when phosphate cleavage and thus ζ potential change was inhibited by phosphatase inhibitor. Conclusion: The developed nanoparticles represent a promising gene delivery system.
Author(s): Prufert F, Bonengel S, Kollner S, Griesser J, Wilcox MD, Chater PI, Pearson JP, Bernkop-Schnurch A
Publication type: Article
Publication status: Published
Journal: Nanomedicine
Year: 2017
Volume: 12
Issue: 22
Pages: 2713-2724
Print publication date: 01/11/2017
Online publication date: 29/09/2017
Acceptance date: 02/04/2016
Date deposited: 08/01/2018
ISSN (print): 1743-5889
ISSN (electronic): 1748-6963
Publisher: Future Medicine Ltd
URL: https://doi.org/10.2217/nnm-2017-0115
DOI: 10.2217/nnm-2017-0115
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