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Lookup NU author(s): Professor Majlinda LakoORCiD, Professor Lyle Armstrong
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© 2017 American Chemical Society. Induced pluripotent stem cells have great potential as a human model system in regenerative medicine, disease modeling, and drug screening. However, their use in medical research is hampered by laborious reprogramming procedures that yield low numbers of induced pluripotent stem cells. For further applications in research, only the best, competent clones should be used. The standard assays for pluripotency are based on genomic approaches, which take up to 1 week to perform and incur significant cost. Therefore, there is a need for a rapid and cost-effective assay able to distinguish between pluripotent and nonpluripotent cells. Here, we describe a novel multiplexed, high-throughput, and sensitive peptide-based multiple reaction monitoring mass spectrometry assay, allowing for the identification and absolute quantitation of multiple core transcription factors and pluripotency markers. This assay provides simpler and high-throughput classification into either pluripotent or nonpluripotent cells in 7 min analysis while being more cost-effective than conventional genomic tests.
Author(s): Baud A, Wessely F, Mazzacuva F, McCormick J, Camuzeaux S, Heywood WE, Little D, Vowles J, Tuefferd M, Mosaku O, Lako M, Armstrong L, Webber C, Cader MZ, Peeters P, Gissen P, Cowley SA, Mills K
Publication type: Article
Publication status: Published
Journal: Analytical Chemistry
Year: 2017
Volume: 89
Issue: 4
Pages: 2440-2448
Online publication date: 11/01/2017
Acceptance date: 11/01/2017
ISSN (print): 0003-2700
ISSN (electronic): 1520-6882
Publisher: American Chemical Society
URL: https://doi.org/10.1021/acs.analchem.6b04368
DOI: 10.1021/acs.analchem.6b04368
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