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Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector

Lookup NU author(s): Professor Robert HirtORCiD

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Abstract

A glucocorticoid-responsive vector is described which allows for the highly inducible expression of complementary DNAs (cDNAs) in stably transfected mammalian cell lines. This vector, pLK-neo, composed of a variant mouse mammary tumor virus long terminal repeat promoter, containing a hormone regulatory element, a Geneticin resistance-encoding gene in a simian virus 40 transcription unit, and a polylinker insertion site for heterologous cDNAs, was used to express the polymeric immunoglobulin (poly-Ig) receptor and the thymocyte marker, Thy-1, in Madin-Darby canine kidney (MDCK) cells and in murine fibroblast L cells. A high level of poly-Ig receptor or Thy-1 mRNA accumulation was observed in MDCK cells in response to dexamethasone with a parallel ten- to 200-fold increase in protein synthesis depending on the recombinant protein and the transfected cell clone.

Publication metadata

Author(s): Hirt RP; Poulain-Godefroy O; Billotte J; Kraehenbuhl JP; Fasel N

Publication type: Article

Publication status: Published

Journal: Gene

Year: 1992

Volume: 111

Issue: 2

Pages: 199-206

Print publication date: 15/02/1992

ISSN (print): 0378-1119

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/0378-1119(92)90687-K

DOI: 10.1016/0378-1119(92)90687-K

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