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Lookup NU author(s): Catriona Manville, Kayleigh Smith, Dr Zbyslaw Sondka, Holly Rance, Dr Ian CowellORCiD, Ka Lee, Dr Nick Morris, Dr Kay Padget, Professor Graham Jackson, Professor Caroline AustinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
We report the whole genome ChIP seq for human TOP2B from MCF7 cells. Using three different peak calling methods, regions of binding were identified in the presence or absence of the nuclear hormone estradiol, as TOP2B has been reported to play a role in ligand-induced transcription. TOP2B peaks were found across the whole genome, 50% of the peaks fell either within a gene or within 5 kb of a transcription start site. TOP2B peaks coincident with gene promoters were less frequently associated with epigenetic features marking active promoters in estradiol treated than in untreated cells. Significantly enriched transcription factor motifs within the DNA sequences underlying the peaks were identified. These included SP1, KLF4, TFAP2A, MYF, REST, CTCF, ESR1 and ESR2. Gene ontology analysis of genes associated with TOP2B peaks found neuronal development terms including axonogenesis and axon guidance were significantly enriched. In the absence of functional TOP2B there are errors in axon guidance in the zebrafish eye. Specific heparin sulphate structures are involved in retinal axon targeting. The glycosaminoglycan biosynthesis-heparin sulphate/heparin pathway is significantly enriched in the TOP2B gene ontology analysis, suggesting changes in this pathway in the absence of TOP2B may cause the axon guidance faults.
Author(s): Manville CM, Smith K, Sondka Z, Rance H, Cockell S, Cowell IG, Lee KC, Morris NJ, Padget K, Jackson GH, Austin CA
Publication type: Article
Publication status: Published
Journal: Biology Open
Year: 2015
Volume: 4
Issue: 11
Pages: 1436-1447
Online publication date: 12/10/2015
Acceptance date: 10/09/2015
Date deposited: 11/01/2016
ISSN (electronic): 2046-6390
Publisher: Company of Biologists Ltd.
URL: http://dx.doi.org/10.1242/bio.014308
DOI: 10.1242/bio.014308
PubMed id: 26459242
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