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Lookup NU author(s): Dr Muhammad Raza, Dr John Perry
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Four isolates of Gram-negative facultatively anaerobic bacteria, three of them producing NDM-1 carbapenemase, were isolated from hospitalized patients and outpatients attending two military hospitals in Rawalpindi, Pakistan, and studied for their taxonomic position. Initially the strains were phenotypically identified as Citrobacter species. Comparative analysis of 16S rRNA gene sequences then showed that the four strains shared >97%, but in no case >98.3%, 16S rRNA gene sequence similarities to members of the genera Citrobacter, Kluyvera, Pantoea, Enterobacter and Raoultella, but always formed a separate cluster in respective phylogenetic trees. Based on multilocus sequence analysis (MLSA) including partial recN, rpoA, thdF and rpoB gene sequence and respective amino acid sequence analysis it turned out that the strains also here always formed separate clusters. Based on further comparative analyses including DNA-DNA hybridizations, genomic fingerprint analysis using rep- and RAPD-PCRs and physiological tests, it is proposed to classify these four strains into the novel genus Pseudocitrobacter gen. nov. with a new species Pseudocitrobacter faecalis sp. nov. with strain 25 CITT (= CCM 8479(T) = LMG 27751(T)) and Pseudocitrobacter anthropi sp. nov. with strain C138(T) (= CCM 8478(T) = LMG 27750(T)), as the type strains, respectively. (C) 2013 Elsevier GmbH. All rights reserved.
Author(s): Kampfer P, Glaeser SP, Raza MW, Abbasi SA, Perry JD
Publication type: Article
Publication status: Published
Journal: Systematics and Applied Microbiology
Year: 2014
Volume: 37
Issue: 1
Pages: 17-22
Print publication date: 01/02/2014
ISSN (print): 0723-2020
ISSN (electronic): 1618-0984
Publisher: Urban und Fischer Verlag
URL: http://dx.doi.org/10.1016/j.syapm.2013.08.003
DOI: 10.1016/j.syapm.2013.08.003
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