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Lookup NU author(s): Sofia Lisanti, Professor Thomas von Zglinicki, Professor John Mathers
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MeDIP (Methylated DNA Immunoprecipitation) is a relatively recent technique aimed to enrich the methylated fraction of DNA with an antibody directed against 5-methyl-cytosine. MeDIP processed samples are suitable for investigation of the methylation status of specific genomic loci and for performing genome-wide screening when hybridized to DNA methylation microarrays or analyzed by deep sequencing. Here, we describe a standardization protocol and quality controls to assess the specificity, reproducibility and efficiency of the MeDIP procedure. These may have utility when comparing results between samples and experiments within laboratories and between laboratories.
Author(s): Lisanti S, von Zglinicki T, Mathers JC
Publication type: Article
Publication status: Published
Journal: Epigenetics
Year: 2012
Volume: 7
Issue: 6
Pages: 615-625
Print publication date: 01/06/2012
ISSN (print): 1559-2294
ISSN (electronic): 1559-2308
Publisher: Landes Bioscience
URL: http://dx.doi.org/10.4161/epi.20028
DOI: 10.4161/epi.20028
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