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Standardization of lymphocyte antibody binding capacity - a multi-centre study

Lookup NU author(s): Alison Bell, Dr Brian Shenton

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Abstract

As quantitative flow cytometry is being increasingly used to characterize non-malignant and malignant disorders, interlaboratory standardization becomes an important issue. However, the lack of standardized methods and process controls with predefined antibody binding capacity values, limits direct interlaboratory comparison. The present study has addressed these issues using a stable whole blood product and a standardized antigen quantification protocol. It was demonstrated that: (i) a standard technical protocol can result in a high degree of interlaboratory concordance; (ii) interlaboratory variation of less than 12% can be achieved for CD4 antibody binding capacity values; and (iii) stable whole blood can be used as a process control with predefined antibody binding capacity values. Furthermore, using such an approach, a normal range was established for CD3, CD4 CD8 and CD19, These antigens appear to be expressed in a hierarchical manner, a factor that could be used as a procedural quality control measure.


Publication metadata

Author(s): Barnett DD, Storie I, Granger V, Whitby L, Reilly JT, Brough S, Garner S, Lawry J, Richards S, Bell AE, Shenton BK

Publication type: Article

Publication status: Published

Journal: Clinical and Laboratory Haematology

Year: 2000

Volume: 22

Issue: 2

Pages: 89-96

ISSN (print): 0141-9854

ISSN (electronic): 1365-2257

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1365-2257.2000.00286.x

DOI: 10.1046/j.1365-2257.2000.00286.x


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