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Apoptotic cells induce dendritic cell-mediated suppression via interferon-γ-induced IDO

Lookup NU author(s): Dr Rachel Harry

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Abstract

Dendritic cells (DC) are sensitive to their local environment and are affected by proximal cell death. This study investigated the modulatory effect of cell death on DC function. Monocyte-derived DC exposed to apoptotic Jurkat or primary T cells failed to induce phenotypic maturation of the DC and were unable to support CD4+ allogeneic T-cell proliferation compared with DC exposed to lipopolysaccharide (LPS) or necrotic cells. Apoptotic cells coincubated with LPS- or necrotic cell-induced mature DC significantly suppressed CD80, CD86 and CD83 and attenuated LPS-induced CD4+ T-cell proliferation. Reduced levels of interleukin-12 (IL-12), IL-10, IL-6, tumour necrosis factor-α and interferon-γ (IFN-γ) were found to be concomitant with the suppressive activity of apoptotic cells upon DC. Furthermore, intracellular staining confirmed IFN-γ expression by DC in association with apoptotic environments. The specific generation of IFN-γ by DC within apoptotic environments is suggestive of an anti-inflammatory role by the induction of indoleamine 2,3-dioxygenase (IDO). Both neutralization of IFN-γ and IDO blockade demonstrated a role for IFN-γ and IDO in the suppression of CD4+ T cells. Moreover, we demonstrate that IDO expression within the DC was found to be IFN-γ-dependent. Blocking transforming growth factor-β (TGF-β) also produced a partial release in T-cell proliferation. Our study strongly suggests that apoptosis-induced DC suppression is not an immunological null event and two prime mediators underpinning these functional effects are IFN-γ-induced IDO and TGF-β. © 2007 The Authors.


Publication metadata

Author(s): Williams CA, Harry RA, McLeod JD

Publication type: Article

Publication status: Published

Journal: Immunology

Year: 2008

Volume: 124

Issue: 1

Pages: 89-101

ISSN (print): 0019-2805

ISSN (electronic): 1365-2567

Publisher: 00192805

URL: http://dx.doi.org/10.1111/j.1365-2567.2007.02743.x

DOI: 10.1111/j.1365-2567.2007.02743.x

PubMed id: 18067553


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