Toggle Main Menu Toggle Search

Open Access padlockePrints

Tissue engineering of tooth crown, root, and periodontium

Lookup NU author(s): Dr Heiko Peters

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Tissue engineering of teeth requires the coordinated formation of correctly shaped crowns, roots, and periodontal ligament. Previous studies have shown that the dental mesenchyme controls crown morphogenesis and epithelial histogenesis during tooth development in vivo, but little is known about the inductive potential of dissociated mesenchymal cells used in ex vivo cultures. A 2-step method is described in which, by using different types of reassociations between epithelial and mesenchymal tissues and/or cells from mouse embryos, reassociations were cultured in vitro before in vivo implantation. In vitro, the reassociated tissues developed and resulted in tooth-like structures that exhibited normal epithelial histogenesis and allowed the functional differentiation of odontoblasts and ameloblasts. After implantation, the reassociations formed roots and periodontal ligament, the latter connected to developing bone. The shape of the crown, initially suspected to depend on the integrity of the mesenchyme, could be modulated by adjusting the number of dissociated mesenchymal cells reassociated with the epithelial compartment. Based on these results, we propose a refined strategy for tooth tissue engineering that may help to eventually generate morphologically defined teeth. © Mary Ann Liebert, Inc.


Publication metadata

Author(s): Hu B, Nadiri A, Kuchler-Bopp S, Perrin-Schmitt F, Peters H, Lesot H

Publication type: Article

Publication status: Published

Journal: Tissue Engineering

Year: 2006

Volume: 12

Issue: 8

Pages: 2069-2075

ISSN (print): 1076-3279

ISSN (electronic): 1937-335X

Publisher: Mary Ann Liebert, Inc.

URL: http://dx.doi.org/10.1089/ten.2006.12.2069

DOI: 10.1089/ten.2006.12.2069

PubMed id: 16968149


Altmetrics

Altmetrics provided by Altmetric


Share