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Lookup NU author(s): Dr James Murray
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The lifetime of a macromolecular crystal in an X-ray beam is assumed to be limited by the absorbed dose. This dose, expressed in Gray (Gy = J kg -1), is a function of a number of parameters: the absorption coefficients of the constituent atoms of the crystal, the number of molecules per asymmetric unit, the beam energy, flux, size and profile, the crystal size, and the total irradiation time. The effects of these variables on the predicted absorbed dose, calculated using the program RADDOSE, are discussed and are illustrated with reference to the irradiation of a selenomethionine protein crystal of unknown structure. The results of RADDOSE can and will in the future be used to inform the data collection procedure as it sets a theoretical upper limit on the total exposure time at a certain X-ray source. However, as illustrated with an example for which the experimental data are compared with prediction, the actual lifetime of a crystal could become shorter in those cases where specific damage breaks down crucial crystal contacts. © 2005 International Union of Crystallography Printed in Great Britain - all rights reserved.
Author(s): Murray JW, Rudino-Pinera E, Owen RL, Grininger M, Ravelli RBG, Carman EF
Publication type: Article
Publication status: Published
Journal: Journal of Synchrotron Radiation
Year: 2005
Volume: 12
Issue: 3
Pages: 268-275
Print publication date: 01/05/2005
ISSN (print): 0909-0495
ISSN (electronic): 1600-5775
Publisher: Wiley-Blackwell
URL: http://dx.doi.org/10.1107/S0909049505003262
DOI: 10.1107/S0909049505003262
PubMed id: 15840910
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