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Helicobacter pylori interacts with the human single-domain trefoil protein TFF1

Lookup NU author(s): Dr Felicity May, Professor Bruce Westley

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Abstract

Why Helicobacter pylori colonizes only gastric tissue is unknown. It is found on gastric mucus-secreting cells and in the overlying gastric mucus but not deep in gastric glands. This localization mirrors the expression of trefoil factor 1, TFF1. We hypothesized that H. pylori interacting with TFF1 could explain the tropism of this bacteria for gastric tissue. Recombinant human TFF1 expressed in Escherichia coli was purified by affinity chromatography, ion-exchange chromatography, and gel filtration. Binding of H. pylori was assessed by using flow cytometry and the BIAcore system, which allows real-time monitoring of molecular interactions. In flow cytometry, H. pylori bound to the TFF1 dimer, but Campylobacter jejuni strains and the laboratory strain of E. coli, HB101, did not bind. When the BIAcore system was used, H. pylori bound strongly to TFF1-coated dextran chips compared with uncoated chips. Binding was inhibited by a TFF1 monoclonal antibody and by soluble TFF1. H. pylori bound to porcine gastric mucin only if it was pretreated with TFF1. In conclusion, H. pylori interacts avidly with the dimeric form of TFF1, and this interaction enables binding to gastric mucin, suggesting that TFF1 may act as a receptor for the organism in vivo. This interaction may underline the previously unexplained tropism of this organism for gastric tissue and its colocalization with the gastric mucin MUC5AC.


Publication metadata

Author(s): Clyne M, Dillon P, Daly S, O'Kennedy R, May F, Westley B, Drumm B

Publication type: Article

Publication status: Published

Journal: Proceedings of the National Academy of Sciences of the United States of America

Year: 2004

Volume: 101

Issue: 19

Pages: 7409-7414

Print publication date: 11/05/2004

ISSN (print): 0027-8424

ISSN (electronic): 1091-6490

URL: http://dx.doi.org/10.1073/pnas.0308489101

DOI: 10.1073/pnas.0308489101

PubMed id: 15123808


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