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A novel copper site in a cyanobacterial metallochaperone

Lookup NU author(s): Dr Gilles Borrelly, Dr Clive Butler, Professor Nigel Robinson

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Abstract

The thylakoid lumen of the cyanobacterium Synechocystis PCC 6803 is supplied with copper via two copper-transporting ATPases anc a metallochaperone intermediary. We show that the copper site of this metallochaperone is unusual and consists of two cysteine residues and a histidine imidazole located on structurally dynamic loops. Substitution of this histidine residue enhances bacterial two-hybrid interaction with the cytosolic copper exporter, but not the copper importer, suggesting that the interacting surfaces are distinct, with implications for metal transfer.

Publication metadata

Author(s): Borrelly GPM, Blindauer CA, Schmid R, Butler CS, Cooper CE, Harvey I, Sadler PJ, Robinson NJ

Publication type: Article

Publication status: Published

Journal: Biochemical Journal

Year: 2004

Volume: 378

Issue: 2

Pages: 293-297

Print publication date: 01/03/2004

ISSN (print): 0264-6021

ISSN (electronic): 1470-8728

Publisher: The Biochemical Society

URL: http://dx.doi.org/10.1042/BJ20031669

DOI: 10.1042/BJ20031669

PubMed id: 14711369

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