Toggle Main Menu Toggle Search

Open Access padlockePrints

The human peroxisome proliferator-activated receptor α gene: Identification and functional characterization of two natural allelic variants

Lookup NU author(s): Dr Surinder Papiha

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Peroxisome proliferator-activated receptor (PPAR)α-null mice have a defect in fatty acid metabolism but reproduce normally. The lack of a detrimental effect of the null phenotype in development and reproduction opens up the possibility for null or variant PPARα gene (PPARA) alleles in humans. To search the coding region and splice junctions for mutant and variant PPARα alleles, the human PPARα gene was cloned and characterized, and sequencing by polymerase chain reaction was carried out. Two point mutations in the human gene were found in the DNA binding domain at codons for amino acids 131 and 162. The allele containing the mutation in codon 162 (CTT to GTT, L162V) designated PPARA*3, was found at a high frequency in a Northern Indian population. Transfection assays of this mutant showed that the non-ligand dependent transactivation activity was less than one-half as active as the wild-type receptor. PPARA*3 was also unresponsive to low concentrations of ligand as compared to the wild-type PPARA*1 receptor. However, the difference is ligand concentration-dependent; at concentrations of the peroxisome proliferator Wy-14 643 > 25 μM, induction activity was restored in this variant's transactivation activity to a level five-fold greater as compared with wild-type PPARA*1 with no ligand. The mutation in codon 131 (CGA to CAA, R131Q), designated PPARA*2 is less frequent than PPARA*3, and the constitutive ligand independent activity was slightly higher than PPARA*1. Increasing concentrations of Wy-14 643 activated PPARA*2 similar to that observed with PPARA*1. The biological significance of these novel PPARα alleles remains to be established. It will be of great interest to determine whether these alleles are associated with differential response to fibrate therapy. (C) 2000 Lippincott Williams and Wilkins.


Publication metadata

Author(s): Sapone A, Peters JM, Sakai S, Tomita S, Papiha SS, Dai R, Friedman FK, Gonzalez FJ

Publication type: Article

Publication status: Published

Journal: Pharmacogenetics

Year: 2000

Volume: 10

Issue: 4

Pages: 321-333

Print publication date: 01/01/2000

ISSN (print): 1744-6872

ISSN (electronic): 1744-6880

Publisher: Lippincott Williams & Wilkins

URL: http://dx.doi.org/10.1097/00008571-200006000-00006

DOI: 10.1097/00008571-200006000-00006

PubMed id: 10862523


Altmetrics

Altmetrics provided by Altmetric


Share