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Lookup NU author(s): Professor Carlos Hormaeche
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In the present study we evaluated the role of B cells in acquired immunity to Salmonella infection by using gene-targeted B-cell-deficient innately susceptible mice on a C57BL/6 background (Igh-6(-/-)). Igh-6(-/-) mice immunized with a live, attenuated aroA Salmonella enterica serovar Typhimurium vaccine strain showed impaired long-term acquired resistance against the virulent serovar Typhimurium strain C5. Igh-6(-/-) mice were able to control a primary infection and to clear the inoculum from the reticuloendothelial system. However, Igh-6(-/-) mice, unlike Igh-6(+/+) C57BL/6 controls, did not survive an oral challenge with strain C5 at 4 months after vaccination. Transfer of immune serum did not restore resistance in Igh-6(-/-) mice. Total splenocytes and purified CD4+ T cells obtained from Igh-6(-/-) mice 4 months after vaccination showed reduced ability to release Th1-type cytokines (interleukin 2 and gamma interferon) upon in vitro restimulation with serovar Typhimurium soluble cell extracts compared to cells obtained from Igh-6(+/+) C57BL/6 control mice. Therefore, the impaired resistance to oral challenge with virulent serovar Typhimurium observed in B- cell-deficient mice, which cannot be restored by passive transfer of Salmonella-immune serum, may be in part due to a reduced serovar Typhimurium- specific T-cell response following primary immunization.
Author(s): Mastroeni P, Simmons C, Fowler R, Hormaeche CE, Dougan G
Publication type: Article
Publication status: Published
Journal: Infection and Immunity
Year: 2000
Volume: 68
Issue: 1
Pages: 46-53
ISSN (print): 0019-9567
ISSN (electronic): 1098-5522
Publisher: American Society for Microbiology
URL: http://dx.doi.org/10.1128/IAI.68.1.46-53.2000
DOI: 10.1128/IAI.68.1.46-53.2000
PubMed id: 10603367
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