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Microassay analyses of protein glycosylation

Lookup NU author(s): Professor Christopher Baldwin

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Abstract

To accurately characterize the carbohydrate moieties of oligosaccharide chains in glycosylated proteins, it is necessary to distinguish exactly which types of oligosaccharides are present at which site. We describe lectin overlay assays, which take advantage of the ability of lectins to distinguish between different types of glycoproteins via recognition of terminal sugars, thus allowing the chain type and peripheral antigenic components to be determined. Three microassays involving lectins are reported in this paper, non-protease-treated intact glycoproteins; glycopeptides released by prior digestion of the glycoprotein and then separated by HPLC; and release of sugars from glycoproteins by hydrazinolysis and then coupling them to a multivalent support.

Publication metadata

Author(s): Wong NKC, Kanu N, Thandrayen N, Rademaker GJ, Baldwin CI, Renouf DV, Hounsell EF

Publication type: Article

Publication status: Published

Journal: Molecular Biotechnology

Year: 2000

Volume: 14

Issue: 2

Pages: 147-155

Print publication date: 01/01/2000

ISSN (print): 1073-6085

ISSN (electronic): 1559-0305

Publisher: Humana Press

URL: http://dx.doi.org/10.1385/MB:14:2:147

DOI: 10.1385/MB:14:2:147

PubMed id: 10872506

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