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Lookup NU author(s): Emeritus Professor Steve Yeaman, Professor Mark Walker
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To investigate the contribution of inherited biochemical defects to the peripheral insulin resistance of type 2 diabetes, we studied cultured skeletal muscle from 10 insulin-resistant nondiabetic first-degree relatives of type 2 diabetic families and 6 control subjects. Insulin stimulation of glucose uptake and glycogen synthesis was maximal in myoblasts. Insulin- stimulated glucose uptake (fold-stimulation over basal uptake) was decreased in relative compared with control myoblasts at 0.001 μmol/l (0.93 ± 0.05 [mean ± SE] vs. 1.15 ± 0.06, P < 0.05) and 0.1 μmol/l (1.38 ± 0.10 vs. 1.69 ± 0.08, P = 0.025) insulin. Insulin responsiveness was markedly impaired in 5 of the relative myoblast cultures, and in 4 of these, there was an associated increase in basal glucose uptake (76.7 ± 7.0 vs. 47.4 ± 5.5 pmol · min-1 · mg-1 protein, relative vs. control; P < 0.02). Expression of insulin receptor substrate 1, phosphatidylinositol 3-kinase, protein kinase B, and glycogen synthase was normal in the relative cultures with impaired insulin responsiveness. Glycogen synthesis was also normal in the relative cultures. We conclude that the persistence of impaired insulin responsiveness in some of the relative cultures supports the role of inherited factors in the insulin resistance of type 2 diabetes and that the association with increased basal glucose uptake suggests that the 2 abnormalities may be linked.
Author(s): Jackson S, Bagstaff SM, Lynn S, Yeaman SJ, Turnbull DM, Walker M
Publication type: Article
Publication status: Published
Journal: Diabetes
Year: 2000
Volume: 49
Issue: 7
Pages: 1169-1177
ISSN (print): 0012-1797
ISSN (electronic): 1939-327X
Publisher: The American Diabetes Association
URL: http://dx.doi.org/10.2337/diabetes.49.7.1169
DOI: 10.2337/diabetes.49.7.1169
PubMed id: 10909975
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