Browse by author
Lookup NU author(s): Dr Oliver Craig, Dr Matthew Collins
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Immunological detection of proteins adsorbed to mineral and ceramic surfaces has proved not only difficult but controversial. Unlike the immunological detection of proteins associated with carbonate or phosphate minerals (e.g. shells and bones) proteins adsorbed to siliceous minerals cannot readily be removed by dissolution of the mineral phase. We have previously examined alternative extraction methodologies which claim to bring the protein into solution, but found none of these to be effective. Here we report a novel strategy for immuno-detection of proteins adsorbed to siliceous minerals, the Digestion and Capture Immunoassay (DACIA). The method involves the use of cold, concentrated (4M) hydrofluoric acid (HF) with the simultaneous capture of liberated protein onto a solid phase. The combination of low temperatures and surface stabilisation enables us to detect epitopes from even partially degraded proteins. The method may have a wide application in forensic, archaeological, soil and earth sciences. Copyright (C) 2000 Elsevier Science B.V.
Author(s): Craig OE, Collins MJ
Publication type: Article
Publication status: Published
Journal: Journal of Immunological Methods
Year: 2000
Volume: 236
Issue: 1-2
Pages: 89-97
Print publication date: 06/03/2000
ISSN (print): 0022-1759
ISSN (electronic): 1872-7905
Publisher: Elsevier Science B.V.
URL: http://dx.doi.org/10.1016/S0022-1759(99)00242-2
DOI: 10.1016/S0022-1759(99)00242-2
PubMed id: 10699582
Altmetrics provided by Altmetric