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Active TGF-β1 expression in kidney transplantation: A comparative study of cyclosporin-A (CyA) and tacrolimus (FK506)

Lookup NU author(s): Dr Helen Robertson, Emeritus Professor John Kirby, David Talbot

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Abstract

Chronic rejection is a major cause of graft dysfunction following kidney transplantation. This fibroproliferative disease may be promoted by overproduction of transforming growth factor beta (TGF-β). Previous studies have suggested that cyclosporin-A (CyA) might increase production of this growth factor. The current study was designed to measure the expression of TGF-β in renal transplant biopsies from patients immunosuppressed with either CyA or tacrolimus. Paraffin-embedded renal biopsies were sectioned, dewaxed and incubated with primary antibody against active TGF-β1 antibody. After washing, the sections were treated with secondary antibody conjugated with fluorescein isothiocyanate (FITC). In each case the sections were assessed by semi-quantitative scanning laser confocal microscopy. Biopsies from patients receiving CyA expressed significantly more active TGF-β1 than biopsies from patients receiving tacrolimus (P < 0.0001, Mann-Whitney test). The increased level of active TGF-β1 expression in renal biopsies of patients receiving CyA may indicate a mechanism of chronic rejection. © Springer-Verlag 2000.

Publication metadata

Author(s): Mohamed MAS, Robertson H, Booth TA, Balupuri S, Gerstenkorn C, Kirby JA, Talbot D

Publication type: Article

Publication status: Published

Journal: Transplant International

Year: 2000

Volume: 13

Issue: S1

Pages: S295-S298

ISSN (print): 0934-0874

ISSN (electronic): 1432-2277

Publisher: John Wiley & Sons

URL: http://dx.doi.org/10.1111/j.1432-2277.2000.tb02042.x

DOI: 10.1111/j.1432-2277.2000.tb02042.x

PubMed id: 11112017

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