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Cellular lysis in Bacillus subtilis; the affect of multiple extracellular protease deficiencies

Lookup NU author(s): Dr Sierd Bron, Professor Colin Harwood

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Abstract

Cellular lysis properties of strains of Bacillus subtilis deficient in the synthesis of extracellular proteases was investigated. In all cases, extracellular protease deficiency was found to increase the extent of cellular lysis of batch cultured strains following the transition to stationary phase, the time at which extracellular degradative enzymes are secreted in large quantities. The data indicates that the major extracellular proteases NprE and AprE, are primarily responsible for the control of this autolytic activity in B. subtilis and has implications for the use of extracellular protease-deficient strains as hosts for the production of heterologous proteins.

Publication metadata

Author(s): Harwood CR; Bron S; Stephenson K

Publication type: Article

Publication status: Published

Journal: Letters in Applied Microbiology

Year: 1999

Volume: 29

Issue: 2

Pages: 141-145

Print publication date: 01/08/1999

ISSN (print): 0266-8254

ISSN (electronic): 1472-765X

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1472-765X.1999.00592.x

DOI: 10.1046/j.1472-765X.1999.00592.x

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