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Surface attachment induced production of antimicrobial compounds by marine epiphytic bacteria using modified roller bottle cultivation

Lookup NU author(s): Professor Grant Burgess

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Abstract

A modified roller bottle culture method elicited the production of antimicrobial compounds from 2 epibiotic marine bacterial strains, EI-34-6 and II-111-5, isolated from the surface of the marine alga Palmaria palmata. These isolates, tentatively identified as Bacillus species, were grown as a biofilm on the surface of nutrient glycerol ferric agar (NGFA) and marine Columbia glycerol agar (MCGA) on the inside of a rolling bottle. The biofilm was shown to be stable, and the cells were difficult to remove from the agar surface. The culture supernatant exhibited a different antibiotic spectrum when the strains were grown using the agar roller bottle method compared with shake flask cultures or nonagar roller bottle cultures. These results suggest that biofilm formation is an important factor in the production of antimicrobial compounds by these 2 strains, and roller bottle cultivation also allowed production of these compounds to be increased. The methodology used here has the potential to allow increased production of useful secondary metabolites such as antibiotics from marine epibiotic bacteria.

Publication metadata

Author(s): Burgess JG; Yan L; Boyd KG

Publication type: Article

Publication status: Published

Journal: Marine Biotechnology

Year: 2002

Volume: 4

Issue: 4

Pages: 356-366

ISSN (print): 1436-2228

ISSN (electronic): 1436-2236

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10126-002-0041-x

DOI: 10.1007/s10126-002-0041-x

PubMed id: 14961247

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