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Lookup NU author(s): Dr Janet Lindsey
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Interest in the fate of long palindromic DNA sequences in E. coli has been kindled by the observation that their inviability is overcome in recBC sbcB strains and that these hosts permit the construction of DNA libraries containing long palindromic sequences present in the human genome. In this paper we show that a reduction in the level of intracellular supercoiled DNA occurs as the result of the presence of a 530 bp palindrome in bacteriophage lambda. This reduction occurs in Rec+ and recA strains but not in strains lacking exonucleases V and I (recBC sbcB). However, the DNA must be active (not repressed) for this reduction to be observed, since it is not seen in a Rec+ host lysogenic for phage lambda. These results argue against two hypotheses: firstly, that the palindrome causes inviability solely by interfering with packaging, and secondly, that it does so solely by interfering with recombination. Conversely, these results suggest that a feature of active monomeric DNA (probably its replication) is involved in inviability.
Author(s): Leach DRF, Lindsey JC
Publication type: Article
Publication status: Published
Journal: Molecular and General Genetics
Year: 1986
Volume: 204
Issue: 2
Pages: 322-327
Print publication date: 01/08/1986
ISSN (print): 0026-8925
ISSN (electronic): 1432-1874
Publisher: Springer Berlin
URL: http://dx.doi.org/10.1007/BF00425517
DOI: 10.1007/BF00425517
PubMed id: PMID: 2945
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