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Lookup NU author(s): Dr Mark EldridgeORCiD
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© 2024. Positron emission tomography (PET) reporter systems are a valuable means of estimating the level of expression of a transgene in vivo. For example, the safety and efficacy of gene therapy approaches for the treatment of neurological and neuropsychiatric disorders could be enhanced via the monitoring of exogenous gene expression levels in the brain. The present study evaluated the ability of a newly developed PET reporter system [18F]fluoroestradiol ([18F]FES) and the estrogen receptor-based PET reporter ChRERα, to monitor expression levels of a small hairpin RNA (shRNA) designed to suppress choline acetyltransferase (ChAT) expression in rhesus monkey brain. The ChRERα gene and shRNA were expressed from the same transcript via lentivirus injected into monkey striatum. In two monkeys that received injections of viral vector, [18F]FES binding increased by 70% and 86% at the target sites compared with pre-injection, demonstrating that ChRERα expression could be visualized in vivo with PET imaging. Post-mortem immunohistochemistry confirmed that ChAT expression was significantly suppressed in regions in which [18F]FES uptake was increased. The consistency between PET imaging and immunohistochemical results suggests that [18F]FES and ChRERα can serve as a PET reporter system in rhesus monkey brain for in vivo evaluation of the expression of potential therapeutic agents, such as shRNAs.
Author(s): Li B, Wadhwa P, Lerchner W, Zanotti-Fregonara P, Liow J-S, Yan X, Zoghbi SS, Nerella SG, Telu S, Morse CL, Solis O, Gomez JL, Holt DP, Dannals RF, Cummins AC, Innis RB, Pike VW, Richmond BJ, Michaelides M, Eldridge MAG
Publication type: Article
Publication status: Published
Journal: Molecular Therapy
Year: 2024
Volume: 32
Issue: 7
Pages: 2223-2231
Print publication date: 03/07/2024
Online publication date: 24/05/2024
Acceptance date: 23/05/2024
ISSN (print): 1525-0016
ISSN (electronic): 1525-0024
Publisher: Cell Press
URL: https://doi.org/10.1016/j.ymthe.2024.05.031
DOI: 10.1016/j.ymthe.2024.05.031
PubMed id: 38796702
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