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Lookup NU author(s): Cassie Bakshani, Dr Ieva Lelenaite, Professor Jeffrey Pearson, Dr Matthew Wilcox, Professor William WillatsORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2024Alginates are abundant linear polysaccharides produced by brown algae and some bacteria. They have multiple biological roles and important medical and commercial uses. Alginates are comprised of D-mannuronic acid (M) and L-guluronic acid (G) and the ratios and distribution patterns of M and G profoundly impact their physiological and rheological properties. The structure/function relationships of alginates have been extensively studied in vitro but our understanding of the in vivo spatiotemporal regulation of alginate fine structures and their biological implications is limited. Monoclonal antibodies (mAbs) are powerful tools for localising and quantifying glycan structures and several alginate-directed mAbs have been developed. We used a library of well-defined alginates, with M and G block ratios determined by NMR, to refine our understanding of the binding properties of alginate-directed mAbs. Using these probes, we obtained new insight into how structural features of alginates are regulated at different scales, from cellular to seasonal.
Author(s): Jones CT, Bakshani C, Lelenaite I, Mravec J, Kracun SK, Pearson J, Wilcox MD, Hardouin K, Kridi S, Herve C, Willats WGT
Publication type: Article
Publication status: Published
Journal: Cell Surface
Year: 2025
Volume: 13
Print publication date: 01/06/2025
Online publication date: 08/12/2024
Acceptance date: 05/12/2024
Date deposited: 13/01/2025
ISSN (electronic): 2468-2330
Publisher: Elsevier B.V.
URL: https://doi.org/10.1016/j.tcsw.2024.100136
DOI: 10.1016/j.tcsw.2024.100136
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