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Lookup NU author(s): Dr Hafez Al-momani, Professor Jeffrey Pearson, Professor Christopher WardORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
© 2024 Al-Momani et al. Introduction: Given the increasing frequency of drug-resistant bacteria and the limited progress in developing new antibiotics, it is necessary to explore new methods of combating microbial infections. Nanoparticles, particularly silver nanoparticles (Ag-NPs), have shown exceptional antibacterial characteristics; however, elevated concentrations of Ag-NPs can produce noticeable levels of toxicity in mammalian cells. Aim: This study examined the potential synergistic effect of combining a low dosage of Ag-NPs and anti-pseudomonas drugs against Pseudomonas aeruginosa (ATCC strain) and eleven clinical isolates from cystic fibrosis patients. Methods: The Ag-NPs were chemically produced by utilizing a seed extract from Peganum Harmala and characterized via ultraviolet-visible spectroscopy and scanning electron microscopy. The broth microdilution technique was utilized to investigate the minimum inhibitory concentration (MIC) of Ag-NPs and eight antibiotics (Piperacillin, Ciprofloxacin, Levofloxacin, Meropenem, Amikacin, Ceftazidime, Gentamicin, Aztreonam). The fractional inhibitory concentration index (FICI) was determined via the checkerboard method to evaluate the synergistic effects of Ag-NPs and various antibiotics. Results: The biosynthesized Ag-NPs were uniformly spherical and measured around 15 nm in size. When combined with antibiotics, Ag-NP produced statistically significant reductions in the amount of antibiotics required to completely prevent P. aeruginosa growth for all strains. The findings revealed that the MIC of Ag-NPs was 15 ug/mL for all strains which decreased substantially when administered with antibiotics at a dose of 1.875–7.5 ug/mL. The majority of Ag-NP and antibiotic combinations exhibited a synergistic or partially synergistic impact. This was particularly noticeable in combinations containing Meropenem, Ciprofloxacin, and Aztreonam (in which the FIC index was less than or equal to 0.5). Conclusion: The findings revealed that combining Ag-NPs with antibiotics was more effective than using Ag-NPs or antibiotics in isolation and that combinations of Ag-NPs and antimicrobial agents displayed synergistic activity against the majority of strains assessed.
Author(s): Al-Momani H, Albalawi H, Al Balawi D, Khleifat KM, Aolymat I, Hamed S, Albiss BA, Khasawneh AI, Ebbeni O, Alsheikh A, Zueter AM, Pearson JP, Ward C
Publication type: Review
Publication status: Published
Journal: International Journal of Nanomedicine
Year: 2024
Volume: 19
Pages: 12461-12481
Online publication date: 23/11/2024
Acceptance date: 01/11/2024
ISSN (print): 1176-9114
ISSN (electronic): 1178-2013
Publisher: Dove Medical Press Ltd
URL: https://doi.org/10.2147/IJN.S479937
DOI: 10.2147/IJN.S479937
PubMed id: 39611007
Data Access Statement: All data generated or analysed during this study are included in this published article and its supplementary information files.
