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Lookup NU author(s): Chaw-Chiea Chang, Dr Yuen Ling Ng, Dr Bernard Loo
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Copyright © 2024 Selvarajan, Teo, Chang, Ng, Cheong, Sivalingam, Khoo, Wong and Loo. Cell and gene therapies are an innovative solution to various severe diseases and unfulfilled needs. Adoptive cell therapy (ACT), a form of cellular immunotherapies, has been favored in recent years due to the approval of chimeric antigen receptor CAR-T products. Market research indicates that the industry’s value is predicted to reach USD 24.4 billion by 2030, with a compound annual growth rate (CAGR) of 21.5%. More importantly, ACT is recognized as the hope and future of effective, personalized cancer treatment for healthcare practitioners and patients worldwide. The significant global momentum of this therapeutic approach underscores the urgent need to establish it as a practical and standardized method. It is essential to understand how cell culture conditions affect the expansion and differentiation of T-cells. However, there are ongoing challenges in ensuring the robustness and reproducibility of the manufacturing process. The current study evaluated various adoptive T-cell culture platforms to achieve large-scale production of several billion cells and high-quality cellular output with minimal cell death. It examined factors such as bioreactor parameters, media, supplements and stimulation. This research addresses the fundamental challenges of scalability and reproducibility in manufacturing, which are essential for making adoptive T-cell therapy an accessible and powerful new class of cancer therapeutics.
Author(s): Selvarajan V, Teo DBL, Chang C-C, Ng YL, Cheong N, Sivalingam J, Khoo SHG, Wong A, Loo BLW
Publication type: Article
Publication status: Published
Journal: Frontiers in Cell and Developmental Biology
Year: 2024
Volume: 12
Online publication date: 12/07/2024
Acceptance date: 25/06/2024
Date deposited: 06/08/2024
ISSN (electronic): 2296-634X
Publisher: Frontiers Media SA
URL: https://doi.org/10.3389/fcell.2024.1427171
DOI: 10.3389/fcell.2024.1427171
Data Access Statement: The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
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