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Lookup NU author(s): Dr Bryan MurchieORCiD, Dr Francesca MullanORCiD, Professor Paula WaterhouseORCiD, Dr Matthew GermanORCiD
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Objectives: 1. To determine the effects of short-term exposure of dentine to different concentrations and pH of citric acid; 2. To determine the stiffness and topographical changes associated with dentine erosion. Methods: Bovine incisors were prepared into 20 dentine discs (5mm3 ) and polished with 0.05µm Al2 O 3 paste. Erosion was measured in terms of topographical and stiffness changes using atomic force microscope (AFM, Nanowizard 3, JPK Instruments). Specimens were exposed via the AFM liquid flow-cell to 1wt% or 6wt% citric acid, either unbuffered (pH 2.17 or 1.88, respectively) or buffered (pH 3.8) for repeated 20s cycles of citric acid up-to 120s. Baseline measured in PBS only. A representative sample was selected from each group for post-erosion scanning electron microscope (SEM) assessment. Results: Table 1 outlines stiffness results and AFM topography images demonstrate all groups post-erosion. The 1% buffered group had the least measurable changes across all time intervals and the 6% unbuffered citric acid had the greatest overall impact on the surface. The 6% buffered acid was more erosive compared with the 1% unbuffered acid, in terms of both stiffness and topography changes, between 20s and 80s exposures. However, the 1% unbuffered group had similar erosive surface changes, as for the 6% buffered samples, with increasing exposure times at 100s onwards. SEM and AFM topography showed a similar loss of peri-tubular dentine with enlargement of the tubules. AFM also demonstrated increased surface height variations with increasing exposure times and similar erosive trends to mechanical stiffness changes. Conclusions: The data shows that citric acid concentration is more critical to early erosive changes on dentine compared with a lower pH, demonstrated by stiffness elasticity reduction and structural changes. The 1% buffered group was the least erosive acid, compared with the 6% unbuffered solution which had the greatest surface changes. These AFM findings were supported by SEM data
Author(s): Murchie B, Mullan F, Waterhouse P, German M
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: British Society for Oral and Dental Research (BSODR) Annual Scientific Meeting 2023
Year of Conference: 2023
Pages: 2-2
Acceptance date: 28/05/2023
Publisher: British Society for Oral and Dental Research
URL: https://www.qmul.ac.uk/dentistry/media/dentistry/new/documents/Abstract-List_143-abstracts.pdf
Notes: https://www.qmul.ac.uk/dentistry/bsodr/abstract-list/
