Toggle Main Menu Toggle Search

Open Access padlockePrints

Differentiation, metabolism and cardioprotective secretory functions of human cardiac stromal cells from ischemic and endocarditis patients

Lookup NU author(s): Dr Annette Meeson, Dr Rachel Oldershaw, Professor Gavin RichardsonORCiD

Downloads


Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

This study investigates the characteristics of cardiac mesenchymal stem cell-like cells (CMSCLCs) isolated from the right atrial appendage of human donors with ischemia and a young patient with endocarditis (NE-CMSCLCs). Typical CMSCLCs from ischemic heart patients were derived from coronary artery bypass grafting procedures, and compared against bone marrow mesenchymal stromal cells (BM-MSCs). NE-CMSCLCs had a normal immunophenotype but exhibited enhanced osteogenic differentiation potential, rapid proliferation, reduced senescence, reduced glycolysis, and lower reactive oxygen species generation after oxidative stress compared to typical ischemic CMSCLCs. These differences suggest a unique functional status of NE-CMSCLCs, influenced by the donor health condition. Despite large variances in their paracrine secretome, NE-CMSCLCs retained therapeutic potential, as indicated by their ability to protect hypoxia/reoxygenation-injured human cardiomyocytes, albeit less effectively than typical CMSCLCs. This research describes a unique cell phenotype and underscores the importance of donor health status in the therapeutic efficacy of autologous cardiac cell therapy.


Publication metadata

Author(s): Nguyen H, Hsu C, Meeson A, Oldershaw RA, Richardson GD, Czosseck A, Lundy D

Publication type: Article

Publication status: Published

Journal: Stem Cells and Development

Year: 2024

Pages: epub ahead of print

Online publication date: 28/06/2024

Acceptance date: 14/06/2024

Date deposited: 02/07/2024

ISSN (print): 1547-3287

ISSN (electronic): 1557-8534

Publisher: Mary Ann Liebert, Inc. Publishers

URL: https://doi.org/10.1089/scd.2024.0103

DOI: 10.1089/scd.2024.0103

ePrints DOI: 10.57711/txdb-tm73

Data Access Statement: All data are contained in the manuscript.

PubMed id: 38940748


Altmetrics

Altmetrics provided by Altmetric


Share