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Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

Lookup NU author(s): Katie Gilmour, Professor Martyn Dade-Robertson

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© The Author(s) 2024. Background: Microbially induced calcium carbonate precipitation has been extensively researched for geoengineering applications as well as diverse uses within the built environment. Bacteria play a crucial role in producing calcium carbonate minerals, via enzymes including carbonic anhydrase—an enzyme with the capability to hydrolyse CO2, commonly employed in carbon capture systems. This study describes previously uncharacterised carbonic anhydrase enzyme sequences capable of sequestering CO2 and subsequentially generating CaCO3 biominerals and suggests a route to produce carbon negative cementitious materials for the construction industry. Results: Here, Bacillus subtilis was engineered to recombinantly express previously uncharacterised carbonic anhydrase enzymes from Bacillus megaterium and used as a whole cell catalyst allowing this novel bacterium to sequester CO2 and convert it to calcium carbonate. A significant decrease in CO2 was observed from 3800 PPM to 820 PPM upon induction of carbonic anhydrase and minerals recovered from these experiments were identified as calcite and vaterite using X-ray diffraction. Further experiments mixed the use of this enzyme (as a cell free extract) with Sporosarcina pasteurii to increase mineral production whilst maintaining a comparable level of CO2 sequestration. Conclusion: Recombinantly produced carbonic anhydrase successfully sequestered CO2 and converted it into calcium carbonate minerals using an engineered microbial system. Through this approach, a process to manufacture cementitious materials with carbon sequestration ability could be developed.


Publication metadata

Author(s): Gilmour KA, Ghimire PS, Wright J, Haystead J, Dade-Robertson M, Zhang M, James P

Publication type: Article

Publication status: Published

Journal: Microbial Cell Factories

Year: 2024

Volume: 23

Online publication date: 10/06/2024

Acceptance date: 23/05/2024

Date deposited: 24/06/2024

ISSN (electronic): 1475-2859

Publisher: BioMed Central Ltd

URL: https://doi.org/10.1186/s12934-024-02437-7

DOI: 10.1186/s12934-024-02437-7

Data Access Statement: All data generated or analysed during this study are included in this manuscript. Further data including source data are available from the corresponding author on reasonable request.

PubMed id: 38858761


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Funding

Funder referenceFunder name
EP/R003629/1EPSRC
EP/R003777/1
Research England E3 scheme (2019)

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