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Bioproduction of high-value raspberry ketone by submerged fermentation of Nidula niveo-tomentosa

Lookup NU author(s): Dr Yi Zhang, Dr Kheng-Lim GohORCiD, Dr Vladimir Zivkovic, Dr Yuen Ling Ng

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2024 Elsevier LtdRaspberry ketone (RK) is a high-value bioactive component in the growing demand for natural compounds in food and flavour industries. However, limited production persists due to low-yield microorganisms and complex biosynthetic pathway. Here, an Nidula niveo-tomentosa fungi-based fermentation system was developed to investigate and optimize the cultivation parameters for natural RK production. While optimizing glucose and phenylalanine concentrations ensured sufficient substrate supply, it unexpectedly induced stress on the substrates. Despite having minimal impact on biomass growth, UV-A radiation effectively boosted RK biosynthesis, but also reaching a ceiling with extended exposure times. Younger precultures exhibited higher specific productivity, older cultures ultimately yielded more RK due to their higher inoculum concentration. Mycelial fungi, compared to fungal pellets, outperformed in RK production, likely facilitated by enhanced nutrient uptake and bioproduct transport. Ultimately, the combination of optimized parameters unlocked a peak RK yield of 154.4 mg/L, underscoring the remarkable potential for natural RK production.


Publication metadata

Author(s): Zhang Y, Goh K-L, Zivkovic V, Ng YL, Chow Y

Publication type: Article

Publication status: Published

Journal: Process Biochemistry

Year: 2024

Volume: 144

Pages: 168-178

Print publication date: 01/09/2024

Online publication date: 31/05/2024

Acceptance date: 30/05/2024

Date deposited: 12/06/2024

ISSN (print): 1359-5113

ISSN (electronic): 1873-3298

Publisher: Elsevier Ltd

URL: https://doi.org/10.1016/j.procbio.2024.05.029

DOI: 10.1016/j.procbio.2024.05.029

ePrints DOI: 10.57711/ehqe-fs51

Data Access Statement: Data will be made available on request.


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Funding

Funder referenceFunder name
Central Public-interest Scientific Institution Basal Research Fund (No. 1610172022014)
Hubei Province Natural Science Foundation of China
National Natural Science Foundation of China
Newcastle University (LOC/150025720/400382711)
SIFBI (under IAFPP3-H20H6a0028)

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