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Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study

Lookup NU author(s): Christine Aldridge

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© Copyright: Introduction: The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods: A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results: A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4% and 85% respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus, Streptococcus species, Enterococcus faecalis, Kingella kingae, Neisseria gonorrhoeae, and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion: The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.

Publication metadata

Author(s): Pascual S, Noble B, Ahmad-Saeed N, Aldridge C, Ambretti S, Amit S, Annett R, O'Shea SA, Barbui AM, Barlow G, Barrett L, Berth M, Bondi A, Boran N, Boyd SE, Chaves C, Clauss M, Davies P, Dianzo-Delgado IT, Esteban J, Fuchs S, Friis-Hansen L, Goldenberger D, Golle A, Groonroos JO, Hoffmann I, Hoffmann T, Hughes H, Ivanova M, Jezek P, Jones G, Ceren Karahan Z, Lass-Florl C, Laurent F, Leach L, Horsboll Pedersen ML, Loiez C, Lynch M, Maloney RJ, Marsh M, Milburn O, Mitchell S, Moore LSP, Moffat L, Murdjeva M, Murphy ME, Nayar D, Nigrisoli G, O'Sullivan F, Oz B, Peach T, Petridou C, Prinz M, Rak M, Reidy N, Rossolini GM, Roux A-L, Ruiz-Garbajosa P, Saeed K, Salar-Vidal L, Salas Venero C, Selvaratnam M, Senneville E, Starzengruber P, Talbot B, Taylor V, Trebse R, Wearmouth D, Willinger B, Wouthuyzen-Bakker M, Couturier B, Allantaz F

Publication type: Article

Publication status: Published

Journal: Journal of Bone and Joint Infection

Year: 2024

Volume: 9

Issue: 1

Pages: 87-97

Online publication date: 28/02/2024

Acceptance date: 01/12/2023

Date deposited: 19/03/2024

ISSN (electronic): 2206-3552

Publisher: Copernicus Publications

URL: https://doi.org/10.5194/jbji-9-87-2024

DOI: 10.5194/jbji-9-87-2024

Data Access Statement: No data sets were used in this article.

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