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Lookup NU author(s): Dr Jinju Chen
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© The Author(s) 2024.Amide herbicides have been extensively used worldwide and have received substantial attention due to their adverse environmental effects. Here, a novel amidohydrolase gene was identified from a soil metagenomic library using diethyl terephthalate (DET) as a screening substrate. The recombinant enzyme, AmiH52, was heterologously expressed in Escherichia coli and later purified and characterized, with the highest activity occurring at 40 ℃ and pH 8.0. AmiH52 was demonstrated to have both esterase and amidohydrolase activities, which exhibited highly specific activity for p-nitrophenyl butyrate (2669 U/mg) and degrading activity against several amide herbicides. In particular, it displayed the strongest activity against propanil, with a high degradation rate of 84% at 8 h. A GC–MS analysis revealed that propanil was transformed into 3,4-dichloroaniline (3,4-DCA) during this degradation. The molecular interactions and binding stability were then analyzed by molecular docking and molecular dynamics simulation, which revealed that several key amino acid residues, including Tyr164, Trp66, Ala59, Val283, Arg58, His33, His191, and His226, are involved in the specific interactions with propanil. This study provides a function-driven screening method for amide herbicide hydrolase from the metagenomic libraries and a promising propanil-degrading enzyme (AmiH52) for potential applications in environmental remediation.
Author(s): Sun S, Chen W, Peng K, Chen X, Chen J
Publication type: Article
Publication status: Published
Journal: Environmental Science and Pollution Research
Year: 2024
Volume: 31
Pages: 20970-20982
Online publication date: 21/02/2024
Acceptance date: 03/02/2024
Date deposited: 01/05/2024
ISSN (print): 0944-1344
ISSN (electronic): 1614-7499
Publisher: Springer
URL: https://doi.org/10.1007/s11356-024-32362-6
DOI: 10.1007/s11356-024-32362-6
Data Access Statement: All the data is included in the manuscript. The raw data is available upon a reasonable request.
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