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Lookup NU author(s): Dr Duy NguyenORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The development of genetic technologies and bioengineering are creating an increasing number of genetically engineered microorganisms with new traits for diverse industrial applications such as vaccines, drugs and pollutant degraders. However, the destiny of genetically engineered bacterial spores released into the environment as long-life organisms has remained a big environmental challenge. In this study, an environmentally responsible and sustainable gene technology solution based on the concept of thymine starvation is successfully applied for cloning and expression of a Helicobacter pylori antigen on Bacillus subtilis spore surface. As an example, a recombinant Bacillus subtilis strain A1.13 has been created from a gene fusion of the corresponding N-terminal fragment of spore coat protein CotB in B. subtilis and the entire urease subunit A (UreA) in H. pylori and the fusion showed a high stability of spore surface expression. The outcomes can open the door for developing highly safe spore vectored vaccines against this kind of pathogen and contributing to reduced potential risks of genetically engineered microorganisms released in the environment.
Author(s): Nguyen TKC, Pham TL, Nguyen TC, Pham TT, Nguyen VD
Publication type: Article
Publication status: Published
Journal: Journal of Pure and Applied Microbiology
Year: 2023
Volume: 17
Issue: 3
Pages: 1605-1611
Print publication date: 01/09/2023
Online publication date: 01/09/2023
Acceptance date: 08/06/2023
Date deposited: 12/02/2024
ISSN (print): 0973-7510
ISSN (electronic): 2581-690X
Publisher: Oriental Scientific Publishing Company, Inc.
URL: https://doi.org/10.22207/JPAM.17.3.21
DOI: 10.22207/JPAM.17.3.21
Data Access Statement: All datasets generated or analysed during this study are included in the manuscript.
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