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Lookup NU author(s): Dr Duy NguyenORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Background: Helicobacter pylori infection remains a major public health threat leading to gastrointestinal illness and increased risk of gastric cancer. Mostly affecting populations in developing countries no vaccines are yet available and the disease is controlled by antimicrobials which, in turn, are driving the emergence of AMR. Materials and Methods: We have engineered spores of Bacillus subtilis to display putative H. pylori protective antigens, urease subunit A (UreA) and subunit B (UreB) on the spore surface. Following oral dosing of mice with these spores, we evaluated immunity and colonization in animals challenged with H. pylori. Results: Oral immunization with spores expressing either UreA or UreB showed antigen-specific mucosal responses (fecal sIgA) including seroconversion and hyperimmunity. Following challenge, colonization by H. pylori was significantly reduced by up to 1-log.ConclusionsThis study demonstrates the utility of bacterial spores for mucosal vaccination to H. pylori infection. The heat stability and robustness of Bacillus spores coupled with their existing use as probiotics make them an attractive solution for either protection against H. pylori infection or potentially for therapy and control of active infection.
Author(s): Katsande PM, Nguyen VD, Nguyen TLP, Nguyen TKC, Mills G, Bailey DMD, Christie G, Hong HA, Cutting SM
Publication type: Article
Publication status: Published
Journal: Helicobacter
Year: 2023
Volume: 28
Issue: 4
Print publication date: 01/08/2023
Online publication date: 14/06/2023
Acceptance date: 23/05/2023
Date deposited: 12/02/2024
ISSN (print): 1083-4389
ISSN (electronic): 1523-5378
Publisher: Wiley-Blackwell Publishing Ltd
URL: https://doi.org/10.1111/hel.12997
DOI: 10.1111/hel.12997
Data Access Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.
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