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Lookup NU author(s): Professor Boguslaw ObaraORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Microscopy image segmentation lays the foundation for shape analysis, motion tracking, and classification of biological objects. Despite its importance, automated segmentation remains challenging for several widely used non-fluorescence, interference-based microscopy imaging modalities. For example in differential interference contrast microscopy which plays an important role in modern bacterial cell biology. Therefore, new revolutions in the field require the development of tools, technologies and work-flows to extract and exploit information from interference-based imaging data so as to achieve new fundamental biological insights and understanding. We have developed and evaluated a high-throughput image analysis and processing approach to detect and characterize bacterial cells and chemotaxis proteins. Its performance was evaluated using differential interference contrast and fluorescence microscopy images of Rhodobacter sphaeroides. Results demonstrate that the proposed approach provides a fast and robust method for detection and analysis of spatial relationship between bacterial cells and their chemotaxis proteins.
Author(s): Obara B, Roberts MA, Armitage JP, Grau V
Publication type: Article
Publication status: Published
Journal: BMC Bioinformatics
Year: 2013
Volume: 14
Online publication date: 23/04/2013
Date deposited: 07/05/2021
ISSN (electronic): 1471-2105
Publisher: BioMed Central Ltd
URL: https://doi.org/10.1186/1471-2105-14-134
DOI: 10.1186/1471-2105-14-134
PubMed id: 23617824
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