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Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1

Lookup NU author(s): Dr Shoba AmarnathORCiD

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Abstract

Immunotherapy using regulatory T cells (Treg) has been proposed, yet cellular and molecular mechanisms of human Tregs remain incompletely characterized. Here, we demonstrate that human Tregs promote the generation of myeloid dendritic cells (DC) with reduced capacity to stimulate effector T cell responses. In a model of xenogeneic graft-versus-host disease (GVHD), allogeneic human DC conditioned with Tregs suppressed human T cell activation and completely abrogated posttransplant lethality. Tregs induced programmed death ligand-1 (PD-L1) expression on Treg-conditioned DC; subsequently, Treg-conditioned DC induced PD-L1 expression in vivo on effector T cells. PD-L1 blockade reversed Tregconditioned DC function in vitro and in vivo, thereby demonstrating that human Tregs can promote immune suppression via DC modulation through PD-L1 up-regulation. This identification of a human Treg downstream cellular effector (DC) and molecular mechanism (PD-L1) will facilitate the rational design of clinical trials to modulate alloreactivity.

Publication metadata

Author(s): Amarnath S, Costanzo CM, Mariotti J, Ullman JL, Telford WG, Kapoor V, Riley JL, Levine BL, June CH, Fong T, Warner NL, Fowler DH

Publication type: Article

Publication status: Published

Journal: PLoS Biology

Year: 2010

Volume: 8

Issue: 2

Online publication date: 02/02/2010

ISSN (print): 1544-9173

ISSN (electronic): 1545-7885

Publisher: Public Library of Science

URL: https://doi.org/10.1371/journal.pbio.1000302

DOI: 10.1371/journal.pbio.1000302

PubMed id: 20126379

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