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Lookup NU author(s): Dr Kaveh Emami
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0).
Two winter triticale (x Triticosecale Wittmack) model cultivars: Hewo (tolerant to pink snow mould) and Magnat (sensitive) were used to test the effect of cold-hardening (4 weeks at 4°C) on soluble ≤50 kDa protein profiles of the seedling leaves. The presence and abundance of individual proteins were analysed via two-dimensional gel electrophoresis (2-DE) and Surface-Enhanced Laser Desorption/Ionization Time-of-Flight (SELDI-TOF). Up to now, no proteomics analysis of triticale response to hardening has been performed. Thus, the present paper is the first in the series describing the obtained results. In our experiments, the exposure to the low temperature-induced only quantitative changes in the leaves of both cultivars, causing either an increase or decrease of 4-50 kDa protein abundance. Among proteins which were cold-accumulated in cv. Hewo's leaves, we identified two thioredoxin peroxidases (chloroplastic thiol-specific antioxidant proteins) as well as mitochondrial- β-ATP synthase subunit and ADP-binding resistance protein. On the contrary, in hardened seedlings of this genotype, we observed the decreased level of chloroplastic RuBisCO small subunit PW9 and epidermal peroxidase 10. Simultaneous SELDI-TOF analysis revealed several low mass proteins better represented in cold-hardened plants of tolerant genotype in comparison to the sensitive one and the impact of both genotype and temperature on their level. Based on those results, we suggest that indicated proteins might be potential candidates for molecular markers of cold-induced snow mould resistance of winter triticale and their role is worth to be investigated in the further inoculation experiments.
Author(s): Golebiowska GJ, Bonar E, Emami K, Wedzony M
Publication type: Article
Publication status: Published
Journal: Acta biochimica Polonica
Year: 2019
Volume: 66
Issue: 3
Pages: 343-350
Print publication date: 17/09/2019
Online publication date: 10/09/2019
Acceptance date: 22/07/2019
Date deposited: 02/10/2019
ISSN (print): 0001-527X
ISSN (electronic): 1734-154X
Publisher: Polskie Towarzystwo Biochemiczne,Polish Biochemical Society
URL: https://doi.org/10.18388/abp.2019_2804
DOI: 10.18388/abp.2019_2804
PubMed id: 31509370
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