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MIP-based biomimetic sensor for the electronic detection of serotonin in human blood plasma

Lookup NU author(s): Professor Marloes PeetersORCiD

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Abstract

Serotonin is an important signaling molecule in the human body. The detection of serotonin is commonly performed by high performance liquid chromatography (HPLC), which is costly and time consuming due to extensive sample preparation. We will show that these problems can be overcome by using molecularly imprinted polymers (MIPs) as synthetic receptors in combination with impedance spectroscopy as readout technique. The MIPs were prepared with several blends of the underlying monomers and the best performing MIP material was selected by optical batch-rebinding experiments. MIP microparticles were then integrated in an impedimetric sensor cell and dose-response curves were measured in PBS buffer and in non-diluted blood plasma. The sensor provides reliable data in the physiologically relevant concentration regime as an independent validation by HPLC measurements demonstrates. Finally, we show that the impedimetric response upon serotonin binding can be attributed to a capacitive effect at the interface between the MIP particles and the plasma. © 2012 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Peeters M, Troost FJ, Van Grinsven B, Horemans F, Alenus J, Murib MS, Keszthelyi D, Ethirajan A, Thoelen R, Cleij TJ, Wagner P

Publication type: Article

Publication status: Published

Journal: Sensors and Actuators B: Chemical

Year: 2012

Volume: 171-172

Pages: 602-610

Print publication date: 01/08/2012

Online publication date: 18/05/2012

ISSN (print): 0925-4005

ISSN (electronic): 1873-3077

Publisher: Elsevier BV

URL: https://doi.org/10.1016/j.snb.2012.05.040

DOI: 10.1016/j.snb.2012.05.040

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