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Lookup NU author(s): Professor Viktor KorolchukORCiD, Dr Bernadette Carroll
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2019 The Author(s).Porcine circovirus type 2 (PCV2) is an economically important swine pathogen but some extra trigger factors are required for the development of PCV2-associated diseases. By evaluating cap protein expression, viral DNA copies and the number of infected cells, the present study further confirmed that oxidative stress can promote PCV2 replication. The results showed that oxidative stress induced autophagy in PCV2-infected PK15 cells. Blocking autophagy with inhibitor 3-methyladenine or ATG5-specific siRNA significantly inhibited oxidative stress-promoted PCV2 replication. Importantly, autophagy inhibition significantly increased apoptosis in oxidative stress-treated PK15 cells. Suppression of apoptosis by benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone in conditions of autophagy inhibition restored PCV2 replication. Taken together, autophagy protected host cells against potential apoptosis and then contributed to PCV2 replication promotion caused by oxidative stress. Our findings can partly explain the pathogenic mechanism of PCV2 related to the oxidative stress-induced autophagy.
Author(s): Zhai N, Liu K, Li H, Liu Z, Wang H, Korolchuk VI, Carroll B, Pan C, Gan F, Huang K, Chen X
Publication type: Article
Publication status: Published
Journal: Veterinary Research
Year: 2019
Volume: 50
Online publication date: 05/03/2019
Acceptance date: 14/02/2019
Date deposited: 20/03/2019
ISSN (print): 0928-4249
ISSN (electronic): 1297-9716
Publisher: BioMed Central Ltd
URL: https://doi.org/10.1186/s13567-019-0637-z
DOI: 10.1186/s13567-019-0637-z
PubMed id: 30836990
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